A silver impregnation method for motor and sensory nerves and their endings in formalin-fixed mammalian muscles.
نویسندگان
چکیده
A silver impregnation method is described which shows motor and sensory nerves and their endings in formalin-fixed mammalian muscles. The method works with the same reliability on flattened muscle pieces as well as on frozen sections. Large nerve bundles, myelinated and non-myelinated single axons, and terminals impregnated by this method stand out black against a light brown background.
منابع مشابه
Identification of sensory neurons supplying receptors in lingual muscles of the rat: histochemical and retrograde labeling study with horseradish peroxidase.
Sensory innervation of lingual musculature was studied in young adult Wistar rats using retrograde labeling by horseradish peroxidase (HRP) and combined silver impregnation and acetylcholinesterase (AchE) methods. Intra-lingual injection of HRP resulted in labeling of neuronal somata in the trigeminal, superior vagal, and second cervical spinal (C2) ganglia. When HRP was directly applied to the...
متن کاملA histochemical study of the cholinesterase activity in the neuromuscular junction in developing chick skeletal muscles.
The development of the motor nerve ending has been studied by many investigators (TELLO, 1922, VISINTINI e LEVI-MONTALCINI, 1939; CUAJUNCO, 1942; KUPFER and KOELLE, 1951) and authors such as GEREBTZOFF (1959), MUMENTHALER and ENGEL (1961), and WAKE (1964) used the chick skeletal muscle to examine the morphogenesis of the ending. However, their studies were confined to the light microscopic obse...
متن کاملLocalization of Nerve Endings in Relation to Cholinesterase Deposits in Normal Human Eye Muscles.
Neroe endings and deposits of cholinesterase were stained in frozen sections of five normal striated human eye muscles with a stain that combines the neroe fiber techniijue of Hortega and the method of Koelle and Friedenwald for the demonstration of cholinesterase. Cholinesterase deposits are found at the base of the motor end plates. Dense cholinesterase deposits can also he found around thin ...
متن کاملReinnervation of muscles after transection of the sciatic nerve in adult rats.
Functional recovery after transection of the sciatic nerve in adult rats is poor, probably because of abnormalities in reinnervation. Denervation and reinnervation patterns were studied morphologically in the lateral gastrocnemius (LGC), tibialis anterior (TA), and soleus (SOL) muscles for 21 weeks after nerve transection (motor endplates by acetylcholinesterase staining; nerves by silver impre...
متن کاملPalisade endings in human extraocular muscles.
The presence of nerve terminals in the tendinous insertions of human eye muscles was investigated histologically in adult human eye muscles obtained from donor eyes and in muscle pieces resected from juvenile patients undergoing surgical correction for strabismus. Lateral and medial recti, superior and inferior oblique muscles from adults, were stained "en bloc" using a silver impregnation meth...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of neuroscience methods
دوره 25 1 شماره
صفحات -
تاریخ انتشار 1988